연구 및 대학원

연구 및 대학원

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대학원 세미나

03/14(목)16시연사:박근완
작성자 관리자조회수 162날짜 2019.03.11
일시: 03월 14일(목) 4:00PM

장소 : 벤처관 711호

연사: 박근완

제목: Computational design of repeat proteins

내용: Repeat proteins have considerable potential for use as modular binding reagents or biomaterials in biomedical and nanotechnology applications. Here we describe a general computational method for building idealized repeats that integrates available family sequences and structural information with Rosetta de novo protein design calculations. Idealized designs from six different repeat families were generated and experimentally characterized; 80% of the proteins were expressed and soluble and more than 40% were folded and monomeric with high thermal stability. Crystal structures determined for members of three families showed high consistency to the design models. The method provides a general approach for fast and reliable generation of stable modular repeat protein scaffolds. The repeat protein idealization method was then used to develop a computational approach to control the shape of the binding surface on leucine-rich repeat (LRR) proteins. A set of self-compatible building block modules were computationally designed that when polymerized each generate surfaces with unique but constant curvatures. In addition, a set of junction modules that connect the different building blocks were designed to precisely adjust the shape of a binding scaffold to match a target of interest. Crystal structures of the designs illustrate the power of the modular approach in controlling repeat protein curvature.
이전 03/21(목)16시연사:김성규
다음 03/07(목)16시연사:김지웅